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Education

Advanced Tissue Culture Technologies - Seminar

Data

Official data in SubjectManager for the following academic year: 2023-2024

Course director

Number of hours/semester

lectures: 0 hours

practices: 0 hours

seminars: 14 hours

total of: 14 hours

Subject data

  • Code of subject: OBH-008-S
  • 1 kredit
  • Biotechnology MSc
  • Specialty in Medical Biotechnology modul
  • autumn
Prerequisites:

OBH-008-E parallel

Exam course:

no

Course headcount limitations

min. 1 – max. 50

Topic

The course material is designed to provide theoretical background to currently employed three dimensional tissue culture techniques including scaffold-based (biodegradable) and scaffold-free (tissue printing, pelleting and hanging-drop) systems. The course will detail the importance of the tissue microenvironment in normal and artificial tissue development. Directed cellular growth, organ formation for surgical implantation and drug target identification in human tissue systems and in vitro testing of pharmaceutical compounds in complex tissue systems are also part of the curriculum. The course will discuss the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions.

Lectures

Practices

Seminars

  • 1. Basic principles of tissue engineering (TE). Characteristics of 2D and 3D cell cultures
  • 2. Cells and tissue types in tissue engineering: isolation, culturing and directed differentiation of different stem cells I.
  • 3. Cells and tissue types in tissue engineering: isolation, culturing and directed differentiation of different stem cells II.
  • 4. Characteristics and use of bioreactors
  • 5. Scaffolds and biomaterials in TE I.
  • 6. Scaffolds and biomaterials in TE II.
  • 7. Scaffold-free technologies used for the generation of aggregate cultures. I.
  • 8. Scaffold-free technologies used for the generation of aggregate cultures. II.
  • 9. Advanced tissue models using transwell inserts: methodology of Air-Liquid Interface (ALI) and Blood-Brain-Barrier (BBB) models.
  • 10. Hepatocyte models for drug induced liver injury I.
  • 11. Hepatocyte models for drug induced liver injury II.
  • 12. Tissue printing technologies I.
  • 13. Tissue printing technologies II.
  • 14. Test

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

Pongracz & Keen: Medical Biotechnology, Elsevier, 2009
Joseph D. Bronzino: Tissue Engineering and Artificial Organs
Ali Khademhosseini (ed.): Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications (Engineering in Medicine & Biology)
Challa S. S. R. Kumar (ed.): Tissue, Cell and Organ Engineering (Nanotechnologies for the Life Sciences)
W. Mark Saltzman: Tissue Engineering: Engineering Principles for the Design of Replacement Organs and Tissues
Robert Lanza, Robert Langer, Joseph Vacanti: Principles of Tissue Engineering, 3rd edition
Will W. Minuth, Raimund Strehl, Karl Schumacher: Tissue Engineering: From Cell Biology to Artificial Organs
Fen Ontaigne: Medicine by Design: The Practice and Promise of Biomedical Engineering, Forgacs & Newman, 2005.

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Every seminar will contain a fast, 10 min test.

Making up for missed classes

None

Exam topics/questions

The list of test questions correlates with the lecture and seminar titles.

Examiners

Instructor / tutor of practices and seminars

  • Dr. Abdelwahab Elhusseiny Mohamed Mahmoud
  • Dr. Bánfai Krisztina
  • Dr. Csöngei Veronika Eszter
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