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Genetic Background of Gene Manipulation II

Data

Official data in SubjectManager for the following academic year: 2023-2024

Course director

Number of hours/semester

lectures: 14 hours

practices: 28 hours

seminars: 14 hours

total of: 56 hours

Subject data

  • Code of subject: OTV-GGM2-T
  • 7 kredit
  • Biotechnology BSc
  • Specialised Core Module modul
  • spring
Prerequisites:

OTV-BIC2-T completed , OTV-GGM1-T completed

Course headcount limitations

min. 5 – max. 50

Topic

The subject expands on the knowledge which was accumulated in the previous semester. Gene mutation, DNA repair mechanisms and transposition, regulation of gene expression, transcriptional and post-transcriptional regulation will be discussed. Finally, epigenetic regulation of gene expression and its consequences will be the final topic.

Lectures

  • 1. Gene mutations - Dr. Pongrácz Judit Erzsébet
  • 2. Replication errors and Base modifications - Dr. Pongrácz Judit Erzsébet
  • 3. Induced mutations induced by chemicals and radiation I. - Dr. Pongrácz Judit Erzsébet
  • 4. Induced mutations induced by chemicals and radiation II. - Dr. Pongrácz Judit Erzsébet
  • 5. Mutations in the background of human diseases I. - Dr. Pongrácz Judit Erzsébet
  • 6. Mutations in the background of human diseases II. - Dr. Pongrácz Judit Erzsébet
  • 7. Transposable elements - Dr. Pongrácz Judit Erzsébet
  • 8. Regulation of gene expression in bacteria - Dr. Pongrácz Judit Erzsébet
  • 9. Regulation of gene expression in Eukaryotes - Dr. Pongrácz Judit Erzsébet
  • 10. Transcriptional regulation in Eukaryotes - Dr. Pongrácz Judit Erzsébet
  • 11. The epigenome - Dr. Pongrácz Judit Erzsébet
  • 12. Regulation of the epigenome - Dr. Pongrácz Judit Erzsébet
  • 13. Epigenetics and diseases - Dr. Pongrácz Judit Erzsébet
  • 14. Epigenetic traits - Dr. Pongrácz Judit Erzsébet

Practices

  • 1. Sequence alignments
  • 2. Sequence alignments
  • 3. Sequence alignments
  • 4. Sequence alignments
  • 5. The effect of UV irradiation on cellular morphology and function
  • 6. The effect of UV irradiation on cellular morphology and function
  • 7. The effect of UV irradiation on cellular morphology and function
  • 8. The effect of UV irradiation on cellular morphology and function
  • 9. The effect of UV irradiation on gene expression
  • 10. The effect of UV irradiation on gene expression
  • 11. The effect of UV irradiation on gene expression
  • 12. The effect of UV irradiation on gene expression
  • 13. BLASTS - bioinformatics
  • 14. BLASTS - bioinformatics
  • 15. BLASTS - bioinformatics
  • 16. BLASTS - bioinformatics
  • 17. Designing disease models - Chromosomal modification - Downsyndrome mouse model
  • 18. Designing disease models - Chromosomal modification - Downsyndrome mouse model
  • 19. Designing disease models - Chromosomal modification - Downsyndrome mouse model
  • 20. Designing disease models - Chromosomal modification - Downsyndrome mouse model
  • 21. Designing disease models - transposons
  • 22. Designing disease models - transposons
  • 23. Designing disease models - transposons
  • 24. Designing disease models - transposons
  • 25. Swiss prot - protein sequences in disease analysis
  • 26. Swiss prot - protein sequences in disease analysis
  • 27. Swiss prot - protein sequences in disease analysis
  • 28. Swiss prot - protein sequences in disease analysis

Seminars

  • 1. Replication errors and Base modifications
  • 2. Replication errors and Base modifications
  • 3. Induced mutations induced by chemicals
  • 4. Induced mutations induced by radiation
  • 5. Mutations in the background of human diseases case study I.
  • 6. Mutations in the background of human diseases case study II.
  • 7. Mutations in the background of human diseases case study III.
  • 8. Comparison of gene expression regulation in bacteria and humans I.
  • 9. Comparison of gene expression regulation in bacteria and humans II.
  • 10. Comparison of gene expression regulation in bacteria and humans III.
  • 11. Comparison of gene expression regulation in bacteria and humans IV.
  • 12. Epigenetics in the background of human diseases case study I.
  • 13. Epigenetics in the background of human diseases case study II.
  • 14. Epigenetics in the background of human diseases case study III.

Reading material

Obligatory literature

Literature developed by the Department

Own ppt, interactive teaching material

Notes

Recommended literature

Klug et al: Concepts of Genetics 12th Edition
Pierce: Genetics - A Conceptual Approach 7th Edition (2020)

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

One midterm test, that can be repeated two more times if necessary.

Making up for missed classes

None

Exam topics/questions

Teams, Neptun, Departmental website

Examiners

  • Dr. Pongrácz Judit Erzsébet
  • Garai Kitti

Instructor / tutor of practices and seminars

  • Ádám Zoltán Mihály
  • Garai Kitti
Rólunk