Industrial Fermentation

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

During the course we employ a set of environmental bacterial strains and use them for various fermentation processes. These bacterial strains include residents of skin and mouth flora, aquatic residents (freshwater and marine), sewage pipeline and agricultural soil residents. We evaluate the ability of such bacterial residents to produce industrially valuable chemicals (on small production scale). We also perform (small scale production) food biotechnology fermentation processes inlcuding the production of cider and yoghurt. General concepts of upscaling and purification, expression systems and bioreactors are also discussed.

Lectures

• 1. Basic concepts of industrial fermentation - Dr. Kvell Krisztián
• 2. Theoretical background of fermentation using environmental bacterial strains - Dr. Kvell Krisztián
• 3. Theoretical background of dextran polymer production by environmental bacterial strains - Dr. Kvell Krisztián
• 4. Theoretical background of protease enzyme production by environmental bacterial strains - Dr. Kvell Krisztián
• 5. Theoretical background of toxin production by environmental bacterial strains - Dr. Kvell Krisztián
• 6. Theoretical background of antibiotic production and antiobotic resistance - Dr. Kvell Krisztián
• 7. Theoretical background of secondary fungal metabolites, their medical use - Dr. Kvell Krisztián
• 8. Theoretical background of cider production, relevant food biotechnology concepts - Dr. Kvell Krisztián
• 9. Theoretical background of cider intermediate product processing - Dr. Kvell Krisztián
• 10. Theoretical background of of cider final product clarification - Dr. Kvell Krisztián
• 11. Theoretical background of cider product evaluation, concepts of gastro-psychology - Dr. Kvell Krisztián
• 13. Theoretical background of yoghurt production, relevant food biotechnology concepts - Dr. Kvell Krisztián
• 13. Theoretical background of yoghurt product evaluation, concepts gastro-psychology - Dr. Kvell Krisztián
• 14. Comparison, basic concenpts of expression systems - Dr. Kvell Krisztián
• 15. Bacterial expression systems - Dr. Kvell Krisztián
• 16. Yeast expression systems - Dr. Kvell Krisztián
• 17. Insect expression systems - Dr. Kvell Krisztián
• 18. Mammalian expression systems - Dr. Kvell Krisztián
• 19. Basic concepts of bioreactor systems - Dr. Kvell Krisztián
• 20. Upscaling, purification, characterization, evaluation using the IMT methodology - Dr. Kvell Krisztián
• 21. Food safety and its relation with industrial fermentation - Dr. Kvell Krisztián
• 22. Food fraud and its relation with industrial fermentation - Dr. Kvell Krisztián
• 23. Food biotechnology and its relation with industrial fermentation - Dr. Kvell Krisztián
• 24. GMOs and their relation with industrial fermentation - Dr. Kvell Krisztián
• 25. Short presentations of students - Dr. Kvell Krisztián
• 26. Short presentations of students - Dr. Kvell Krisztián
• 27. Summary - Dr. Kvell Krisztián
• 28. Test - Dr. Kvell Krisztián

Practices

• 1. Introduction to industrial fermentation terminology, basic concepts in the lab
• 2. Technical attributes of propagation, production using environmental bacterial strains
• 3. Production of dextran polymer by Streptococcus mutans of the oral cavity
• 4. Production of protease enzyme by various environmental resident bacterial strains
• 5. Production of hemolyzing toxin by various environmental resident bacterial strains
• 6. Production of antibiotic and observation of antiobotic resistance
• 7. Long-term fungal (yeast) cultures, evaluation of secondary metabolites
• 8. Initiation of cider production
• 9. Sedimentation of cider intermediate product
• 10. Clarification of cider final product using enzymatic cocktail
• 11. Cider production and evaluation, practical gastro-psychology
• 12. Yoghurt production and evaluation, practical gastro-psychology
• 13. Demonstration of bioreactor system utilization, practical notes
• 14. Summary, discussion of practice lab notes

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

1. Practical Manual on Fermentation Technology by S. Kulandaivel & S. Janarthanan, I K International Publishing House, 2012, ISBN-13: 978-9381141809
2. Fermentation and Biochemical Engineering Handbook by Celeste M. Todaro and Henry C. Vogel, William Andrew, 2014, ISBN-13: 978-1455725533
3. Practical Fermentation Technology by Brian McNeil and Linda Harvey, Wiley, 2008, ISBN-13: 978-0470014349

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Written test

Making up for missed classes

None

Exam topics/questions

www.medbiotech.com

Examiners

• Dr. Kvell Krisztián

Instructor / tutor of practices and seminars

• Dr. Kvell Krisztián