Basics in Cell and Tissue Cultures

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

The course material discusses the basic principles of cell culture techniques both for cell lines and primary tissues. Fundamental techniques for stem cell purification and stem cell culture will also be detailed. The course will provide both theoretical and practical background for the use of tissue cultures in complex pharmaceutical testing and biotechnological modification of cells and tissues.

Lectures

Practices

• 1. Practicing sterile work in a tissue culture laboratory, thawing up adhesive and non-adhesive cell lines
• 2. Keeping cell lines in culture, passage of cell cultures, counting and pelletting cells, setting up cell cultures at set cell numbers
• 3. Storage and freezing down cell lines.
• 4. Practicing microbial screening I.
• 5. Practicing microbial screening II.
• 6. Testing cell lines for transdifferentiation, apoptosis, etc.. General fingerprinting of cell lines
• 7. Designing immortalization of primary cells
• 8. Purification of primary cells using tissue characteristic markers
• 9. Culturing primary cells and testing survival and apoptotic death
• 10. Purification of adult stem cells based on predicted markers
• 11. Culturing stem cells
• 12. Testing for differentiation markers and cellular proliferation
• 13. Presentation and discussion: Individual experiments designed by students
• 14. Presentation and discussion: Individual experiments designed by students

Seminars

• 1. History of cell cultures and introduction to a cell culture facility
• 2. Cell and tissue types in culture. Origins of cell types, tissue banks
• 3. Regulations on using cell cultures
• 4. Cell culture protocols, risk assessment and microbial screening
• 5. Preparatory techniques used in creating cell lines from complex tissues
• 6. Differentiation and redifferentiation of cell lines. Testing spontaneous transformation. Regular fingerprinting
• 7. Arteficially immortalized cell types
• 8. Experimental designs using cell cultures
• 9. Main characteristics of stem cells and origin of stem cell types
• 10. Stem cell markers and stem cell cultures
• 11. Keeping stem cells in culture
• 12. Directed differentiation of stem cells
• 13. Complex stem cell cultures
• 14. Experimental designs using stem cells

Reading material

Obligatory literature

Literature developed by the Department

ppt

Notes

Basic and Complex cell and Tissue Culture techniques for Medical Biotechnology and Biotechnology MSc Students, PTE, 2014

Recommended literature

Bernice M. Martin: Fundamental Techniques in Cell Culture - a Laboratory Handbook (ECACC?), Tissue Culture Techniques: An Introduction, paperback, Aug 1, 1994
Kerry Atkinson: Clinical Bone Marrow and Blood Stem Cell Transplantation, 3rd edition, Cambridge University Press, 2004
Leda dos Reis Castilho: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Routledge, 2008
Clive Svendsen, Svendsen, Allison D. Ebert: Encyclopedia of Stem Cell Research, Sage eReference, SAGE Publications, 2008

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Written exam

Making up for missed classes

None

Exam topics/questions

Neptun MeetStreet, Teams

Examiners

Instructor / tutor of practices and seminars

• Bóvári-Biri Judit
• Dr. Bánfai Krisztina
• Garai Kitti